Access to imaging technologies and expertise in the National Microscopy Infrastructure is provided by the five NMI Nodes. The Nodes offer a powerful portfolio of different state-of-the-art technologies in the fields of biological imaging. Each Node brings in a different combination of technologies as well as their expertise with distinct applications and methods.
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Multimodal assimilation of technologies provides complementary and often unique information, spatial, structural, biochemical and biophysical details from the same target sample.
Super-resolution Structured illumination microscopy (SIM)
SIM microscopy system can produce twice the resolution of conventional optical microscopes
Single molecule localization microsopy
Within the NMI infrastructure we have the equipment and experience to performe single molecule localization microscopy with both PALM and STORM.
STED – Stimulated emission depletion microscopy
At the NMI infrastructure we have the equipment and experience to perform STED in a diversity of sample preparations.
CLEM – Correlative light electron microscopy
Equipment and experience to perform CLEM using transmission electron microscopy (TEM) and scanning electon microscopy (SEM)
CLM-AFM – Correlative Light and Atomic Force Microscopy
Equipment and experience to perform CLM-AFM
High throughput confocal microscopy
We enable large number of fixed samples to be imaged in 96 or 384 plate format using laser scanning confocal microscopy.
FCS/FCCS – Fluorescence correlation spectroscopy
Measures diffusion coefficients and concentrations of fluorescent molecules.
RESOLFT – Reversible Saturable Optical Fluorescence Transitionsmicroscopy
The RESOLFT technique is currently under development at the KTH node.
Light sheet microscopy
Permits optical sectioning of large samples, with little to no phototoxicity or photobleaching and high temporal resolution.
IVM – Intravital microscopy
NMI offer a broad range of intravital microscopy applications with multi- and/or single-photon microscopy and FLIM.
Cryo electron microscopy
Equipment and experience to perform Cryo EM.