Access to imaging technologies and expertise in the National Microscopy Infrastructure is provided by the five NMI Nodes. The Nodes offer a powerful portfolio of different state-of-the-art technologies in the fields of biological imaging. Each Node brings in a different combination of technologies as well as their expertise with distinct applications and methods.
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Multimodal microscopy
Multimodal assimilation of technologies provides complementary and often unique information, spatial, structural, biochemical and biophysical details from the same target sample.
![](https://usercontent.one/wp/nmi.brismar.se/wp-content/uploads/2021/09/ana_sim_meiosis.jpg)
Super-resolution Structured illumination microscopy (SIM)
SIM microscopy system can produce twice the resolution of conventional optical microscopes
![](https://usercontent.one/wp/nmi.brismar.se/wp-content/uploads/2021/09/Image-197_PALM_2-1024x637.jpg)
Single molecule localization microsopy
Within the NMI infrastructure we have the equipment and experience to performe single molecule localization microscopy with both PALM and STORM.
![](https://usercontent.one/wp/nmi.brismar.se/wp-content/uploads/2021/04/image-1.jpeg)
STED – Stimulated emission depletion microscopy
At the NMI infrastructure we have the equipment and experience to perform STED in a diversity of sample preparations.
![](https://usercontent.one/wp/nmi.brismar.se/wp-content/uploads/2021/04/image-2.jpeg)
CLEM – Correlative light electron microscopy
Equipment and experience to perform CLEM using transmission electron microscopy (TEM) and scanning electon microscopy (SEM)
![](https://usercontent.one/wp/nmi.brismar.se/wp-content/uploads/2021/04/image-2.png)
CLM-AFM – Correlative Light and Atomic Force Microscopy
Equipment and experience to perform CLM-AFM
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High throughput confocal microscopy
We enable large number of fixed samples to be imaged in 96 or 384 plate format using laser scanning confocal microscopy.
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FCS/FCCS – Fluorescence correlation spectroscopy
Measures diffusion coefficients and concentrations of fluorescent molecules.
![](https://usercontent.one/wp/nmi.brismar.se/wp-content/uploads/2021/09/IMG_1256-1024x768.jpg)
RESOLFT – Reversible Saturable Optical Fluorescence Transitionsmicroscopy
The RESOLFT technique is currently under development at the KTH node.
![](https://usercontent.one/wp/nmi.brismar.se/wp-content/uploads/2021/04/image-5.png)
Light sheet microscopy
Permits optical sectioning of large samples, with little to no phototoxicity or photobleaching and high temporal resolution.
![](https://usercontent.one/wp/nmi.brismar.se/wp-content/uploads/2021/04/image-9-e1632334146808.png)
IVM – Intravital microscopy
NMI offer a broad range of intravital microscopy applications with multi- and/or single-photon microscopy and FLIM.
![](https://usercontent.one/wp/nmi.brismar.se/wp-content/uploads/2021/04/image-6.png)
Cryo electron microscopy
Equipment and experience to perform Cryo EM.