Access to imaging technologies and expertise in the National Microscopy Infrastructure is provided by four NMI Nodes. The Nodes offer a powerful portfolio of different state-of-the-art technologies in the fields of biological imaging. Each Node brings in a different combination of technologies as well as their expertise with distinct applications and methods.
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Multimodal microscopy
Provides information, spatial, structural, biochemical and biophysical details from the same target sample.
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Super-resolution Structured illumination microscopy (SIM)
Can produce twice the resolution of conventional optical microscopes.
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Singel molecule localization microsopy
Equipment and experience to perform single molecule localization microscopy with both PALM and STORM.
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CLEM – Correlative light electron microscopy
Equipment and experience to perform CLEM using transmission electron microscopy (TEM) and scanning electon microscopy (SEM)
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CLM-AFM – Correlative Light and Atomic Force Microscopy
Equipment and experience to perform CLM-AFM
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High throughput confocal microscopy
We enable large number of fixed samples to be imaged in 96 or 384 plate format using laser scanning confocal microscopy.
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FCS/FCCS – Fluorescence correlation spectroscopy
Measures diffusion coefficients and concentrations of fluorescent molecules.
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RESOLFT – Reversible Saturable Optical Fluorescence Transitionsmicroscopy
The RESOLFT technique is currently under development at the KTH node.
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Light sheet microscopy
Permits optical sectioning of large samples, with little to no phototoxicity or photobleaching and high temporal resolution.
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STED – Stimulated emission depletion microscopy
Equipment and experience to perform STED in a diversity of sample preparations.
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IVM – Intravital microscopy
NMI offer a broad range of intravital microscopy applications with multi- and/or single-photon microscopy and FLIM.
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Cryo electron microscopy
Equipment and experience to perform Cryo EM.